It first appears in the level "Induction", when it is used by the United States Marine Corps during the Battle of Seoul. It is used again in "Atlas", where Mitchell has to get the President of the United States inside the vehicle to escape Camp David. The vehicle is finally controlled by the player in the level "Collapse". After the player gets on the Golden Gate Bridge, however, their Pitbull flips and they must continue on foot. The United States Marine Corps' version of the Pitbull can one again be seen in the background of the opening cutscene of "Throttle".
In the campaign mission Bio Lab, Gideon and Mitchell must sneak past a Seeker variant of the Pitbull. A Seeker is similar to the Pitbull, but has a beam used for disabling Exo-cloaks. The beam is a color blue, and when spotting a Exo-cloak, turns red.